wild-type cells

And those healthy, wild-type cells are the final strain involved.

Their differentiation was therefore compared to that of the wild-type cells.

Importantly however, the disappearance of all three proteins was clearly delayed by 48 hours compared to wild-type cells (compare Figs.

Since the sensitivity of our Rum1p sera was insufficient to detect the low levels present in wild-type cells (see Fig.

These double auxotrophs served as the recipient in the study, wild-type cells (Trp+ , His+ ) were the donors.

Finally, wee1 mutant fission yeast cells are smaller than wild-type cells, but take just as long to go through the cell cycle.

In wild-type cells, at 11 hours after wounding, repair of the wound was essentially complete with the denuded area covered with fibroblasts (Fig.

In agreement with this, the RARβ2 mRNA is fully induced by day 3 in wild-type cells.

In lines A and B, induction was seen only after 5 days and this induction was transient compared to wild-type cells.

Experimental evidence also suggests that gene expression can oscillate with the period seen in dividing wild-type cells independently of the CDK machinery.

This is because the wild-type cells are better at adhering and more effectively combine into aggregates; knockout (KO) cells are left behind.

Activation of CD47 with TSP-1 in wild-type cells inhibits proliferation and reduces expression of stem cell transcription factors.

Indirect immunofluorescence staining indicated that endogenous Myc-tagged Pcu3p is present in the cytoplasm, but enriched in the nucleus of wild-type cells (Fig.

Therefore, wild-type cells do not grow on YP sucrose plates that contain 2-DG, due to glucose repression of SUC2.

This is a big help if the first strain is around, but these proteins perform helpful functions, so these cells can also be outcompeted by healthy, wild-type cells.

This defect in cell motility was rescued to the level of wild-type cells by the addition of purified SPARC (Fig.

The majority of asci from wild-type cells contained four well-rounded spores, each with a single DAPI-staining body of equal intensity (Figure 3A).

Each of the rec12 mutants behaved similarly and produced approximately 10% diploid meiotic products, more than 30-fold higher than the frequency produced by wild-type cells (Figure 4B).

As a reference for SCF Popcomplex formation, we used strains carrying 13Myc epitope-tagged Pop1p or Pop2p integrated into the genome of wild-type cells.

Mixtures of csA knockout cells with wild-type cells yield spores, "born" from the fruiting bodies, which are 82% wild-type (WT).

However, it was later found that WHI1 was in fact a positive regulator of Start, as its deletion caused cells to delay in G1 and grow larger than wild-type cells.

We used reverse transcription coupled-PCR (RT-PCR) to compare the induction of some of these genes in lines A and B with that observed in wild-type cells.

However, SUTs can be observed in both Δrrp6 mutants and wild-type cells, indicating they are only partially degraded by the exosome and are able to escape the Nrd1-Nab3-Sen1 pathway.

However, E. coli mutants that lack an active electron transport chain produced as much hydrogen peroxide as wild-type cells, indicating that other enzymes contribute the bulk of oxidants in these organisms.

The pattern of PPT1 gene expression was identical in wild-type cells and cells expressing HA-Ppt1p, indicating that the epitope tag did not alter transcription or message stability (data not shown).