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Similar results were obtained at 7 days post transfection (Figure 9).
A minimum of 50 cells were examined for each transfection.
Numbers in parentheses give the times every transfection was repeated.
The cells were washed and collected 48 h after transfection.
The word transfection is a blend of trans- and infection.
Another practical application of the compound is its use in gene transfection.
This is a quick and easy method for transfection, albeit a rather inefficient one.
This process is also referred to as transfection or transduction, respectively.
Dendrosomes: a novel family of vehicles for transfection and therapy.
The ΔC2 cell line was from the first transfection method and all of the others were selected using the second.
Cells were harvested for reporter assays 48 hours after transfection.
Each transfection was repeated at least three times.
The 4003 oligonucleotides were introduced into the tumour cells by transfection.
Whole-cell recordings and immunostaining were done 3-5 days after transfection.
Incubation with radioisotope was then performed 48 h after transfection.
Differences in transfection efficiency were normalized taking the β-gal values as control.
The procedure for the transfection of an interleukin-4 gene into a mousepox virus is described.
The meaning of the term transfection has evolved.
Flow cytometry revealed significant increase in green fluorescence upon transfection with anti-miR-92.
Therefore, the transfections were repeated multiple times to control for variability in transfection efficiency.
Transfection is the process of deliberately introducing nucleic acids into cells.
The material may become trapped in the nucleus following mitosis and never reach stable transfection.
Transfection is typically done with viral vectors, such as retroviruses.
Twenty-four h before transfection, cells were trypsinized and transferred to 12-well plates.
The resulting β-galactosidase activity was used to normalize transient transfection results.