A target-specific probe, composed of 20 oligonucleotide pairs, hybridizes to the target RNA(s).

The intensity of these bands is related to the amount of the target RNA in the samples analyzed.

This recognition sequence is bipartite (constructed from the two different arms of the loop region) and forms complex pseudo-knots with the target RNA.

It has no identified target RNA.

The pseudouridylation pocket ensures modification of the correct uridine, as it contains two short sequences that are complementary to the target RNA.

The same membrane can be probed up to five times without a significant loss of the target RNA.

Target-specific probes hybridize to each target RNA.

A target-specific probe set containing 20 oligonucleotide pairs hybridizes to the target RNA.

Each fully assembled amplification structure is contained within 40 50 bp of target RNA with the capacity for 400-fold signal amplification.

It is slower and less quantitative, but also produces accurate information about the size of the target RNA.