supernatant fluid

(v) Remove as much of the supernatant fluid as possible.

The supernatant fluid is tested for agglutinating activity for guinea pig erythrocytes.

Discard the supernatant fluid and resuspend the sedimented cells in approximately 3 ml of 1% sodium citrate solution.

These are the most universal character and are used for various purposes - from preparing solutions and decanting supernatant fluids to simple reactions.

Supernatant fluid was collected immediately after infection and washing to establish that any unattached CMV was not present.

After incubation at 37 C for 48 h, the culture supernatant fluids were removed and the cells washed once with PBS.

When the tubules appear "flat" and opaque allow them to settle, and carefully transfer the supernatant fluid into a 15 ml centrifuge tube.

The amount of gp120 in the virus pellets and the supernatant fluids was calculated from calibration curves relating absorbance readings to gp120 dilutions.

The pellet was collected for protein assay while ACh was extracted by mixing the supernatant fluid with chloroform and water (1:2:1 v/v).

TcdA and TcdB are present in supernatant fluids of Clostridium difficile cultures and can be purified from filtrates.

The virus containing pellets and supernatant fluids were assayed by an ELISA allowing gp120 determination in the presence of CAP.

Invitro tests that are sensitive to wheat gluten found that tryptic peptides of avenin could not induce EMA production in supernatant fluid from cultured duodenal mucosa specimen from celiac patients.

A drop of the clear supernatant fluid was placed on an agar plate, which had previously been thickly planted with M. lysodeikticus, and the plate was incubated at 37 C. for 24 hours, when it showed a copious growth of the coccus, except in the region where the nasal mucus had been placed.