The supernatant liquid was centrifuged for 20 minutes at 19500 g.

He waited for the action to go to completion and then poured off the supernatant liquid.

The supernatant water is then run back into the treatment process or disposed of as a waste-water stream.

The supernatant liquid (containing many of the fission products) was separated from the solid.

(v) Remove as much of the supernatant fluid as possible.

The albumin remains in the supernatant fraction during the solid/liquid extraction under these conditions.

Increased lysosomal fragility is indicated by an increase in supernatant enzyme.

During the fourth stage the outlet valve opens and the "clean" supernatant liquor exits the tank.

The remaining solution is properly called the "supernate" or "supernatant liquid".

On top of the sand bed sits a supernatant layer of raw, unfiltered water.

They should instead stay in the liquid above the crystals (chemists call this the "supernatant liquid").

Retroviral supernatant samples were directly loaded onto the membrane without further preparation.

PA317-infected cells were grown in selection media for 2 weeks and pooled for supernatant collection.

After 5 minutes, decant carefully the supernatant solun.

The supernatant fluid is tested for agglutinating activity for guinea pig erythrocytes.

Additional promising preliminary research demonstrates that removal of supernatant plasma from stored transfusions may have beneficial effects.

The formation of the cellulose pellicle occurs on the upper surface of the supernatant film.

The supernatant solution was either used immediately or stored at -20 C prior to RIA.

After centrifugation the supernatant solution is removed, leaving a pellet of crude DNA.

The supernatant liquid is then either quickly decanted from the tube without disturbing the precipitate, or withdrawn with a Pasteur pipette.

The two supernatant fractions were combined and centrifuged for 30 min at 27,000 x g to spin down the brush border membrane vesicles.

These are the most universal character and are used for various purposes - from preparing solutions and decanting supernatant fluids to simple reactions.

We concentrated viral supernatant 100-fold in as few as four hours by centrifugation at 20,000 g , with complete recovery of infectious viral particles.

The homogenate was centrifuged at 100,000 x g for 30 minutes at 4 C and the supernatant fraction (cytosol) was collected.

When the tubules appear "flat" and opaque allow them to settle, and carefully transfer the supernatant fluid into a 15 ml centrifuge tube.

The supernatant was stored at -20 C until further use.

The supernatant was removed and centrifuged at 100 g for five minutes.

Then the supernatant in each well can be checked for desired antibody.

After the final wash, remove as much supernatant as possible.

Following centrifugation, the supernatant was aspirated and saved for analysis.

Physical methods to concentrate viral supernatants have been pursued with mixed results.

In all cases, serial dilutions of viral supernatant were tested.

Cancer cell line supernatant is an attractive source of secreted proteins.

The supernatants were removed, and placed on ice.

After centrifuging the samples at 1500 rpm for five minutes the supernatant was discarded.

The suspension is allowed to rest for a period of time, after which the supernatant is carefully poured off.

There is only one justified way to perform this dilution - by using equilibrium supernatant.

Supernatants were taken at 5, 10, and 15 days and stored at -20 C for later analysis.

Samples were then centrifuged at 13,000 rpm for 2 min and the supernatant was discarded.

The supernatant was collected and the procedure repeated.

Cell culture supernatants and cells were harvested and the virus concentrated.

Place drops of the clear supernatant on to dental wax in a Petri dish as before.

Discard most of the supernatant, leaving behind just enough to resuspend the cells.

To avoid protein contamination, the supernatant on the top is carefully removed without disturbing the interface.

The toxin concentration of the supernatant is proportional to the organism cell count.

If the precipitate forms, decant the supernatant and use as the indicator solution.

They can easily be transfected either with viral supernatant or physical methods.

On the 2 ndday, culture supernatants were removed from each well and fresh medium was added.

This final supernatant was the nuclear extract.

Tubes were centrifuged at 17000 g for five minutes and the supernatants were recovered.