The distance from the known restriction site to the DNase cut is then measured to give the location.

Typically, a restriction site will be a palindromic sequence of about four to six nucleotides long.

There are hundreds of restriction endonucleases known, each attacking a different restriction site.

On the one hand, restriction sites can be generally represented by consensus sequences.

If the mutation creates or destroys a restriction site then this can be simply examined in the products of the reaction.

There are 6 key restriction sites inside the amp gene.

The restriction sites may be further used for sub-cloning into another vector if necessary.

This method has an advantage over other gene splicing techniques in not requiring restriction sites.

If two clones, they will likely have restriction sites in common, and will thus share several fragments.

Only relevant restriction sites are indicated in the expanded regions.