Assays were carried out using a radiolabelled restriction fragment containing the anti- met- box construct (00186).

A subset of the restriction fragments is then selected to be amplified.

Typically 50-100 restriction fragments are amplified and detected on denaturing polyacrylamide gel.

Through restriction fragment length polymorphism analysis, the two strains have been shown to differ only slightly.

A single mammalian cell only provides two copies of any given restriction fragment, which can be ligated to only one other partner in 3C.

There are two common mechanisms by which the size of a particular restriction fragment can vary.

Subcloning of specific restriction fragments were performed as described by Johansen (50).

The black boxes indicate the locations of the binding sites in the vectors and restriction fragments used for bending assays.

That was ordinary enough, but the writer of this letter also made it clear he had used a computer "to sort through the restriction fragments."

The desired restriction fragments were purified by agarose gel electrophoresis.