These media significantly increase the cost of producing recombinant proteins.
Ideally, the recombinant protein can be directly purified from the culture medium.
In general, fungi are excellent hosts for the production of recombinant proteins.
The first human recombinant protein used for disease management was insulin and it was introduced in 1982.
We typically obtained 100-300 μg of recombinant protein from 10 9Sf9 cells.
The trial was designed for three patients because we have only a small amount of the recombinant protein, which took more than two years to produce.
Since 1992 recombinant proteins are generally used, which contain little, if any, human blood products - and thus have a negligible risk of contamination.
A vaccine based on a recombinant protein is available.
Genetic systems have also been developed which allow the production of recombinant proteins using E. coli.
This approach could be useful as an alternative to frequent injections of recombinant protein into the eye.