With this design, when the probe hybridizes to the target, it undergoes an inversion in configuration (as suggested by the name of the technique) and circularizes.

Since each probe hybridizes to exactly one SNP target in the genome, successfully circularized probes provide the nucleotide identities of the SNPs.

The correct probe hybridizes, is ligated, rinsed and the fluorescence is read and recorded.

Experimentally, one can attempt to solve the problem by hybridizing a clone (as probe) to genomic DNA (target) from both species and determining to which target the probe hybridizes.

A target-specific probe, composed of 20 oligonucleotide pairs, hybridizes to the target RNA(s).

Probe size is important because longer probes hybridize less specifically than shorter probes.

In its simplest form, a fluorescently labelled probe hybridizes to its complementary sequence adjacent to the primed template.

Target-specific probes hybridize to each target RNA.

When a probe hybridizes to an open hairpin, complete refolding of the hairpin is stalled, and the position of the hybridized probe can be inferred.

They found that nearly all probes on the ear array hybridized to cDNA prepared from endosperm or ear, whereas the endosperm array contained many apparently tissue-specific elements.