The phosphate groups bind to the 5' carbon of the sugar.
This is because the phosphate group from the reduction process are needed for carbon fixation.
A phosphate group is transferred to the sugar, and Asp-186 may be deprotonated by water.
The ionic nature is found in the salt bridge formed from Arg359 to the phosphate group.
Note that the phosphate group does not have to come directly from the substrate.
We won't take on a phosphate group, so it will be helpless and will probably release us.
In doing so, it gained a phosphate group and energy.
I then neutralized the ship and the phosphate group fell off.
The process of removing the phosphate group is called dephosphorylation.
FK-506 seemed like "a great tool" to find out what phosphate groups do to different proteins.