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Image analysis artifacts result in a higher noise rate for the oligonucleotide probes.
This bias has since been corrected in more recent oligonucleotide array designs.
It seems likely that a failure in oligonucleotide probe design was responsible for the discordant data.
The numbering scheme for the top strand of the oligonucleotide is shown below.
It is an artificial oligonucleotide named for being a mirror image of natural oligonucleotides.
To furnish a functional oligonucleotide, all the protecting groups have to be removed.
Each cell (also called a probe) in the array contains the same oligonucleotide sequences.
There are several stages at which binding of the oligonucleotide could inhibit virus production.
This simple transformation often accounted for much of the variability between oligonucleotide arrays.
In each case we observed consistent oligonucleotide performance.
Each reaction contained 0.5ng of 5' end labelled single or double stranded oligonucleotide probe.
An oligonucleotide probe test is very accurate but is not available in all labs.
The necessary primer optimization should be performed using specialized oligonucleotide design programs.
Here, traditional, linear oligonucleotide probes failed to yield results.
For oligonucleotide arrays, there are similar and sometimes more complicated issues for the same steps.
An example oligonucleotide array signal distribution and quantile comparison can be seen in Figure 1.
The location and direction of oligonucleotide primers used in the analysis are shown as arrowheads.
This was also true for oligonucleotide 3.
The group is widely used in oligonucleotide synthesis.
An oligonucleotide containing the desired mutation is used for primer extension.
For a more comprehensive review, see oligonucleotide synthesis.
Each oligonucleotide is designed to be either part of the top or bottom strand of the target sequence.
Low specificity of probes is also a frequently encountered problem in oligonucleotide arrays.
These approaches may also be applied to pairs of single-channel oligonucleotide arrays.
The sequence and location of each oligonucleotide is available online [ 23].