oligonucleotide synthesis

The group is widely used in oligonucleotide synthesis.

For a more comprehensive review, see oligonucleotide synthesis.

In the past, oligonucleotide synthesis was carried out manually in solution or on solid phase.

This very feature makes nucleoside phosphoramidites useful intermediates in oligonucleotide synthesis.

The FlexArrayer is an in-house custom oligonucleotide synthesis instrument.

The amino group is then used as an anchoring point for linkers suitable for oligonucleotide synthesis (see below).

Automated synthesis originated with peptide and oligonucleotide synthesis.

Before the first oligonucleotide synthesis step the complete DNA microarray surface is covered by photolabile groups.

The main method is currently by oligonucleotide synthesis (also used for other applications) from digital genetic sequences and subsequent annealing of the resultant fragments.

With decreasing costs of oligonucleotide synthesis, artificial gene synthesis is now occasionally used as an alternative to site-directed mutagenesis.

Oligonucleotides are readily biotinylated in the course of oligonucleotide synthesis by the phosphoramidite method using commercial biotin phosphoramidite.

In the early 1950s, Alexander Todd's group pioneered H-phosphonate and phosphate triester methods of oligonucleotide synthesis.

DMT group is widely used for protection of 5'-hydroxy group in nucleosides, particularly in oligonucleotide synthesis.

Oligonucleotide synthesis is the chemical synthesis of relatively short fragments of nucleic acids with defined chemical structure (sequence).

The evolution of oligonucleotide synthesis saw four major methods of the formation of internucleosidic linkages and has been reviewed in the literature in great detail.

The newly formed tricoordinated phosphite triester linkage is not natural and is of limited stability under the conditions of oligonucleotide synthesis.

With respect to the chemistry, synthesis of oligonucleotide microarrays is different from the conventional oligonucleotide synthesis in two respects:

Because of the inherent difficulties of oligonucleotide synthesis, the proportion of full-length (25 mer) probes within a feature is rather low [ 1].

Isolation of nucleic acids, gel electrophoresis, hybridization conditions, oligonucleotide synthesis and standard in vitro recombinant techniques were carried out as described elsewhere (27, 28).

In the routine oligonucleotide synthesis, exocyclic amino groups in nucleosides are kept permanently protected over the entire length of the oligonucleotide chain assembly.

Oligonucleotide synthesis is carried out by a stepwise addition of nucleotide residues to the 5'-terminus of the growing chain until the desired sequence is assembled.

The major derivatives are fluorescein isothiocyanate (FITC) and, in oligonucleotide synthesis, 6-FAM phosphoramidite.

TBDMS and TOM groups are used for protection of 2'-hydroxy function in nucleosides, particularly in oligonucleotide synthesis.

The nucleic acids themselves are then synthesized using standard oligonucleotide synthesis methods, usually automated in an oligonucleotide synthesizer, and strands of custom sequences are commercially available.

In oligonucleotide synthesis, several phosphoramidite reagents containing protected fluorescein, e.g. 6-FAM phosphoramidite 2, are widely used for the preparation of fluorescein-labeled oligonucleotides.