The mode of action appears to be nuclease activity resulting in the induction of apoptosis.
Treatment with a protease removed the 'forward' nuclease activity of this enzyme.
The nuclease activity of Dna2 is required for removing these long flaps, leaving a shorter flap to be processed by Fen1.
A cloned version of this enzyme without any nuclease activity is also available.
Mre11 has nuclease activity, but it seems to be involved in homologous recombination, not NHEJ.
The recD mutant does not show nuclease activity but is recombination-proficient and able to repair the broken DNA molecules [ 6 ] .
- note that the nuclease activity reported here is an artifact.
Vent polymerase lacking nuclease activity [ 15 ] is also expected to give a low noise level.
It is possible that the nuclease activity of telomerase causes the erosion.
SAMHD1 also has nuclease activity.