In particular, they are often "fed" using mouse embryonic fibroblasts ("feeder cells") while being simultaneously suspended in a nutrient solution ("media").
Using a chemical technique in the laboratory, the team transplanted a gene for human growth hormone into mouse fibroblasts growing in laboratory cell cultures.
PtdIns(3,5)P2 was first reported to occur in mouse fibroblasts and budding yeast S. cerevisiae in 1997.
In 2006, he and his team generated induced pluripotent stem cells (iPS cells) from adult mouse fibroblasts.
Upon induction, mouse fibroblasts must undergo MET to successfully begin the initiation phase of reprogramming.
-on feeder layers of mouse embryonic fibroblasts that have been gamma-irradiated to prevent their replication.
Furthermore the hapten-binding particles were able to transfer the neo gene and the antibody-envelope fusion gene to mouse fibroblasts.
This indicates that the virus particles bind specifically to hapten, are infectious and can package a marker gene for transfer into mouse fibroblasts.
In 2006, Yamanaka and his team took adult mouse fibroblasts and turned them back into stem cells in tissue culture.
CYR61 was first identified as a protein encoded by a serum-inducible gene in mouse fibroblasts.