The somatic form is expressed in all mitotic cells, is localized to the nucleus, and is coupled to the cell cycle.
If possible, siliconizing of equipment should be avoided since any carry over of silicon can severely inhibit the spreading of mitotic cells.
The aim should be to score between 50 and 100 mitotic cells.
Real mitotic cells can be visualized through the microscope by staining them with fluorescent antibodies and dyes.
While mitotic cells have condensed chromosomes, interphase cells do not.
In a mitotic cell wherever two asters convene the formation of a spindle occurs.
Labelling with an anti-phospho-histone 3 antibody did not reveal any significant changes in the number of mitotic cells (not shown).
In this way, the spindle in a mitotic cell has two poles emanating microtubules.
This stops cell division at mitosis which allows an increased yield of mitotic cells for analysis.
Homologous chromosomes of a dividing, mitotic cell contain complementary telomeric sequences.