Trunk fragments with attached limbs (+AER and -AER) were cultured as described in the legend to Fig. 1 for 48h in serum-free medium supplemented as stated (resupplemented after 24h).

Thus, digital painting is not so much a new medium as a new appearance of the whole range of existing mediums, supplemented with some new features.

Thus, after microinjection, the cells were grown in medium supplemented with serum for 4 to 6 hours and then serum starved overnight to cause the redistribution of GR-GFP.

When confluent cells were used as donors, fused embryos were cultured in the medium supplemented with 7.5 μg/ml cytochalasin B to prevent extrusion of polar bodies.

Cell lines were grown in Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum, except for MCF10A cells, which were grown as recommended by the ATCC.

Cultures were founded from -80 freezer isolates and grown in Davis minimal (DM) medium supplemented with 1000 μg/ml of glucose.

HL-60 proliferates continuously in suspension culture in nutrient medium supplemented with fetal bovine serum, L-glutamine, HEPES and antibiotic chemicals.

Monolayers of human hepatoma cell line Hep3B were maintained in Dulbecco modified Eagle's medium supplemented with 10% fetal calf serum.

Cell cycle analysis Cells were plated at 2 x 10 5/ml in specific medium supplemented as above.

Stable infectants were selected in medium supplemented with 1 mg/ml G418 and 15% BCL 1 .3B3-conditioned medium.