Genome blot analysis Genomic DNA (5-10 μg) was digested with restriction enzymes according to the manufacturers recommendations.

Unfortunately, some manufacturers recommendations about appropriate sizes for particular spaces have not been sufficiently precise to guarantee that ozone concentrations will not exceed public health limits.

Cells were transfected with Lipofectamine 2000 reagent according to the manufacturers recommendations (Invitrogen).

Vehicles with large and wide low profile tires are more prone to the effects as well as vehicles which have wheels fitted that are larger than the manufacturers recommendation or have reinforced sidewalls.

Dilution This usually occurs when the dilution of the paint is not done as per manufacturers recommendation.

Planned maintenance is created for every item separately according to manufacturers recommendation or legislation.

Carbon monoxide detectors/alarms should always be installed as per manufacturers recommendations where a wood stove is in use.