In molecular biology, the choloylglycine hydrolase family is a family of linear amide C-N hydrolases which cleave carbon-nitrogen bonds, other than peptide bonds, in linear amides.

This enzyme belongs to the family of hydrolases, those acting on carbon-nitrogen bonds other than peptide bonds, specifically in linear amides.

Due to ring strain, β-lactams are more reactive to hydrolysis conditions than are linear amides or larger lactams.