The geometrical/linear optical theory, to account at the first approximation for the spatial intensity distribution.
Here the UV laser is split into two beams which interfere with each other creating a periodic intensity distribution along the interference pattern.
The filter is used to modify the intensity distribution in a radiation beam.
Step 2: using the values for all the genes in the list, generate integral intensity distributions for both experiments.
The intensity distribution in the images depends on the phase shifts inserted by the micro-objects under study.
The actual sampled resolution will depend on the spot size and intensity distribution.
For electron gun scanning systems, the spot usually has a Gaussian intensity distribution.
The following images show the radial intensity distribution of the simulated Arago spot images above:
These methods use the heterogeneity in the intensity distribution of fluorescence to measure the molecular brightness of different species in a sample.
The third column is the light intensity distribution in a beam cross-section (with a dark vortex core at the center).