All mice homozygous for these Pten mutations die during early embryogenesis.

Fewer than expected homozygous mutant mice survived until weaning.

However, no homozygous yellow mice were obtained.

The quarter that died were the homozygous yellow mice that Cuénot did not see in his tests.

Less homozygous mutant mice survived to weaning than expected.

Fewer than expected homozygous mutant mice were identified at weaning.

The remaining tests were carried out on both heterozygous and homozygous mutant adult mice.

Male homozygous mice also had abnormal indirect calorimetry measures.

Hemizygous males were infertile and thus it was not possible to produce homozygous mutant female mice.

The homozygous mutant mice were profoundly deaf, showing no cochlear responses.