They derived a relationship between the observed fluorescence intensity and the distance of the fluorophore from a reflective silicon surface.

Quenching refers to any process which decreases the fluorescence intensity of a given substance.

The slow decrease of the fluorescence intensity at later times can be caused, in addition to other processes, also by non-photochemical quenching.

At low concentrations the fluorescence intensity will generally be proportional to the concentration of the fluorophore.

The change in fluorescence intensity of the sensing films resulting is directly related to the saccharide concentration.

This can lead to the inaccurate measurement of fluorescence intensity.

The fluorescence intensity was constant over a range of several μm above and below the focal plane (data not shown).

A computer program measures the results, determining the quantity or size of the fragments, at each data point, from the level of fluorescence intensity.

Using Image-1 software, regions were defined over single cells and the average fluorescence intensity of the region was quantified.

The fluorescence intensity is fluctuating due to Brownian motion of the particles.