When embryonic stem cells are grown in the absence of feeder cells, extracellular matrix components such as diluted Matrigel are necessary to maintain the pluripotent, undifferentiated state (self-renewal).
Mouse embryonic fibroblasts (MEFs) are often used as "feeder cells" in human embryonic stem cell research.
In particular, they are often "fed" using mouse embryonic fibroblasts ("feeder cells") while being simultaneously suspended in a nutrient solution ("media").
The flat elongated cells are fibroblasts used as "feeder cells."
In addition, adipose-derived stem cells from both human and animals reportedly can be efficiently reprogrammed into induced pluripotent stem cells without the need for feeder cells.
Many of the world's cell lines, including the Goteborg ones, are grown on mouse cell mediums, beds of "feeder cells" that secrete a signal that prevents embryonic cells from maturing.
Prior to each cytotoxicity assay, clones underwent ficoll-hypaque centrifugation to remove dead feeder cells and were determined to be greater than 80% CD8+ tetramer-positive T cells by FACS.
Prior to each experiment, Transwell inserts with confluent MGBE were transferred to new plates without feeder cells.
The first stem cells were cultivated on a layer of feeder cells that provided them the right kind of surface to grow on.
This derived P19 cells grew rapidly without feeder cells and was easy to maintain.