It is this enzymatic step that requires vitamin C as a cofactor.

For example, consider an enzymatic step with a kinase, phosphatase, and substrate.

In the final enzymatic step, the glycosidic bond is cleaved through reaction with a phosphate group, yielding α-D-glucose-1-phosphate.

Stereochemistry of the peptide segments are defined by the substrate active-site in each enzymatic step.

The biosynthesis process often consists of several enzymatic steps in which the product of one step is used as substrate in the following step.

Glycosylation of the proteoglycan occurs in the Golgi apparatus in multiple enzymatic steps.

Unsaturated and odd-chain fatty acids require additional enzymatic steps for degradation.

The precise sequence of the intermediate enzymatic steps is not known with certainty, and the biosynthetic pathway may differ between species.

The biosynthetic pathway to paclitaxel has been investigated and consists of approximately 20 enzymatic steps.

Indeed, glycosylation is thought to be the most complex post-translational modification, because of the large number of enzymatic steps involved.