deoxyribonucleotide

The systematic name of this enzyme class is deoxyribonucleotide 3'-phosphohydrolase.

Each deoxyribonucleotide comprises three parts: a nitrogenous base, a deoxyribose sugar, and one phosphate group.

The millimolar concentration of deoxyribonucleotide triphosphate.

A deoxyribonucleotide is the monomer, or single unit, of DNA, or deoxyribonucleic acid.

Unlike the other deoxyribonucleotide triphosphates, thymidine triphosphate does not contain the "deoxy" prefix in its name.

Activated free deoxyribonucleotides exist in the cell as deoxyribonucleotide triphosphates (dNTPs).

The lack of the 3' hydroxyl group terminates the chain reaction as the DNA polymerases mistake it for a regular deoxyribonucleotide.

Ribonucleotide reductase, a key enzyme in DNA synthesis, catalyses the de novo production of deoxyribonucleotide precursors.

This enzyme reduces the 2' hydroxyl group on the ribose sugar to deoxyribose, forming a deoxyribonucleotide (denoted dATP).

It therefore cannot bond with the next base and terminates the chain, as DNA polymerases cannot distinguish between it and a regular deoxyribonucleotide.

A microwell containing a template DNA strand to be sequenced is flooded with a single species of deoxyribonucleotide triphosphate (dNTP).

The process is similar in DNA biosynthesis, except that ATP is reduced to the deoxyribonucleotide dATP, before incorporation into DNA.

Biochemical research as a DNA replication inhibitor that causes deoxyribonucleotide depletion and results in DNA double strand breaks near replication forks (see DNA repair)

In nature, the incorporation of a deoxyribonucleotide triphosphate (dNTP) into a growing DNA strand involves the formation of a covalent bond and the release of pyrophosphate and a positively charged hydrogen ion.

The metal ions are general divalent cations that help the 3' OH initiate a nucleophilic attack onto the alpha phosphate of the deoxyribonucleotide and orient and stabilize the negatively charged triphosphate on the deoxyribonucleotide.