Different organisms have cyst walls made of different things.
The new cyst wall is thin, transparent, and easily ruptured.
Here it becomes spherical and develops a cyst wall around itself.
As compared to the other forms, it is generally smaller in size and has a thick multilayered cyst wall.
But because the cyst walls are lined with nerves, this may not be an option.
On rare occasions, colpoda have been observed to divide into 4 individuals without producing a cyst wall.
Gomori methenamine-silver method stains the cyst wall brown or black.
The composition of the cyst wall is variable in different organisms.
The cyst wall is formed by the parasitophorous vacuole membrane.
The interior of the cyst is removed followed by the cyst wall.