Consistent with the inhibition of actin polymerization in the permeabilized cell assay, cells treated with BDM do not show a brightly staining actin network at the leading edge [Figure 2A,2B].

The advantage of this method is that the concentrations of compounds in these extracts is sometimes too low to be identified in whole cell assays but are easily found in this two-plate assay system.

Mechanism of action Chemical epistasis studies We sought to determine the level at which the agonist acts in the Hh pathway, in cultured cell assays.

Some of the benefits of using a secreted reporter molecule like MetLuc is its no-lysis protocol that allows one to be able to conduct live cell assays and multiple assays on the same cell.

Rhodamine fluorescence can also be used as a measure of membrane polarization in live cell assays both within mitochondria and with bacteria.

Two weeks later Starzl flew to Japan, where Fujisawa gave him less than a gram of the material - enough for cell assays and some experiments in rats.

Using GFP-tagged NPCs, we designed live cell assays to monitor NPC dynamics and assembly rates [ 25 ] .

Many cell assays have been developed to assess specific parameters or response of cells (biomarkers, cell physiology.

Discussion In this report, we describe a convenient cell culture-based assay for assessing the contributions made by candidate components of Wnt signaling pathways to the activation of the β-catenin-mediated branch.

Further studies reproduced the results of FAS I inhibition as the putative mechanism first in whole cell assay of replicating M. tuberculosis bacilli which have shown that pyrazinoic acid and its ester inhibit the synthesis of fatty acids.