The Irish assay system is based on that of the UK.
In 1987, the assay system was privatized and since 1988 has been located in only an office at Gouda.
The magnitude of the enteroglucagon response elicited by deoxycholate was similar to that reported for a test breakfast, measured using the same assay system.
The different target cells and the assay systems used might explain the discrepancy.
Using this assay system, we screened 140,000 synthetic compounds at a concentrations of 2-5 μM and isolated several putative agonists.
To specifically test this, we used two established in vitro assay systems that detect the effects of Hh on primary neuronal precursors.
Enteroglucagon was measured by an assay system.
Phosphatidylserine (20 g/ml) and Ca + (free concentration, 10 -mol/l) were also present in the assay system.
DMDA showed no effects in any of these assay systems.
Proteins can be detected down to femtomole quantities, well below the detection limit for most assay systems.