As more THC was added to the cells, the response to the glycine increased in magnitude.
Still, he said, the fact that the technique involves adding genes to the cells adds some uncertainty.
Sodium chloride is added to the outside cells to replace what has been electrolyzed.
Then he added genes for an immune system hormone, tumor necrosis factor, to the cells.
In order to prepare a slide for cytogenetic study, a mitotic inhibitor is added to the cells being studied.
Following virus adsorption (2 h), compounds in growth medium were added to the cells.
The Effectene-DNA complexes are mixed with the medium and directly added to the cells.
This media containing compounds (500 μL) was then added to the cells.
The transfection solution (800 l) was added to the cells which were cultured for 16hrs, washed and re-fed.
The suspension of the precipitate is then added to the cells to be transfected (usually a cell culture grown in a monolayer).