Several efforts were made to generate rabbit monoclonal antibodies after the development of mouse hybridoma technology in the 1970s.
Research was conducted into mouse-rabbit hetero-hybridomas to make rabbit monoclonal antibodies.
Rabbit antibodies against the gp41 six-helix bundle were prepared as described [ 21 ] .
Another agglutination test mixes the blood being tested with a sheep's red blood cells that have been covered with rabbit antibodies.
Therefore rabbit antibodies against a whole cell sonicated lysate of M. paratuberculosis were affinity purified.
Cell line 240E-W has since been further developed and optimized for production of rabbit monoclonal antibodies for research and commercial applications.
Cell lysates were incubated with 2 μg of rabbit polyclonal antibody overnight at 4 C on a rotator.
A rabbit polyclonal anti-hsp90 (H-114) antibody (Santa Cruz) diluted 1:1000 was used as a control.
Lobar pneumonia could be treated with antiserum, an injection of rabbit antibodies against the pneumococcus, if the intern identified the subtype correctly.
Alfred Nisonoff's most important work started when he began the enzymatic cleavage of rabbit antibodies to better determine the structure that contributed to their specificity in disease.