Equal amounts of tissues were pooled from each time point for RNA isolations.
Equal amounts of tissue were pooled from each time point for RNA isolation.
Microarrays are sensitive to noise from many sources including the manufacturing process and the experimental (RNA isolation, labeling, hybridization, staining, washing and scanning) processes.
TCEP is also used in the tissue homogenization process for RNA isolation.
In this study, the HEL cells were incubated with the inoculum for 5 days before the cultures were processed for RNA isolation.
RNA isolation Total RNA was prepared from each sample (eight normal kidneys, 13 tumors).
It might be useful for increasing efficiency of RNA isolation.
Thus, the left gastrocnemius was harvested for RNA isolation and Northern analyses in six rats.
Woman: I also enjoy working on RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction.
At 40 to 48 h after the first transfection, cells were photographed, or processed for immunostaining (see below), RNA isolation, or immunoblot, as and where indicated.