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- The deduced amino acid sequence can be saved in various formats and searched against protein databases using BLAST.
- A variety of tools are available for searching the SNP database, allowing search by genotype, method, population, submitter, markers and sequence similarity using BLAST.
- Orthologs were found all the way back to the scarlet sea anemone and homologs were found in bacteria and Archaea using BLAST.
- The basic level of annotation is using BLAST for finding similarities, and then annotating genomes based on that.
- Sequence identity and similarity were determined using BLAST with the reference human sequence as the query.
- The below table shows some orthologs found using BLAST.
- We then carried out additional sequence analyses of these genes using BLAST, Pfam [ 21] and COG to confirm our prediction.
- The below table shows some, but not all, orthologs which were found using BLAST.
- To determine if it might be a BDNF 5' exon it was compared with all known sequences using BLAST.
- We used BLAST to search annotated repeats against the rice repeat database, using an E-value cutoff of 10 -8.
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