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There may be vacuolated cells in the upper portion of the prickle cell layer.
Those coccidian parasites, which are spore-forming, single-celled microorganisms, initially establish themselves in large vacuolated cells of the host's digestive gland, where they reproduce freely.
Time-lapse tracking in Norway Spruce Picea abies revealed that neither single cytoplasmic-rich cells nor vacuolated cells developed into embryos.
He recognized under the name of primordial utricle the protoplasmic lining of the vacuolated cell, and first described the behaviour of the protoplasm in cell division.
Proembryogenic masses (PEMs), an intermediate between unorganized cells and an embryo composed of cytoplasmic-rich cells next to a vacuolated cell, are stimulated with auxin and cytokinin.
The salt gland continues to develop to produce two to four vacuolated cells at the level of the epidermis, the stalk cell with an almost completely cutinized wall, and at least eight terminal cells.