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Both methods require an ultraviolet microscope as protein absorbs light at 280nm.
Ultraviolet microscopes have two main purposes.
Barnard's achievement was to develop the ultraviolet microscope (invented in Germany in 1903) for the study of viruses.
Techniques for surpassing the resolution limit for bright field microscopy include ultraviolet microscopes, which use shorter wavelengths of light so the diffraction limit is lower.
Ultraviolet microscopes and UV enhanced optics must be used when a UV laser source is used for Raman microspectroscopy.
He collaborated with his friend Conrad Beck, the optical manufacturer, in applying these improvements to a production model, and the Beck-Barnard instrument, the first really efficient ultraviolet microscope, was placed on the market in 1929.
When viewed through an ultraviolet microscope, the nicked, noncancerous cells appear to have a fluorescent rim, while cancerous cells have little or no halo, according to Dr. Puck, who said the test produced the same results with 17 types of cancer cells.