trypsinization

Trypsinization is often used to passage cells to a new vessel.

When trypsinization process is complete the cells will be in suspension and appear rounded.

Even with a hot room, it is sometimes convenient to have another incubator close to the hood for trypsinization.

Removal of cells was done by trypsinization.

Western blotting Cells were harvested by trypsinization and the cell number was determined using a hemacytometer.

The ability of anti-CD45-coated beads to bind to CD45 + cells is not prevented by prior trypsinization (our unpublished observations).

The drug resistant cells were harvested by trypsinization, pooled, and cloned to single cells by limiting dilution in 96 well plates.

The cells were harvested by trypsinization, sonicated, and incubated for 1 hr in 10% tri-chloro-acetic acid (TCA) on ice.

Subconfluent cultures were collected by trypsinization, washed with ice cold PBS and lysed by three freeze/thaw cycles in sterile deionized water.

In brief, rat pituitary cells at 72 h after adeno-RGS3 infection (5 M.O.I.) were collected by trypsinization, homogenized, and a protein determination made.

Growing RASMCs were harvested by trypsinization and washed in DMEM + BSA as described above.

For preparation of donors in G0/G1 phase, the cells were allowed to grow to confluency by culturing for 6 days and a single cell suspension was prepared by standard trypsinization.

Trypsinization is the process of cell dissociation using trypsin, a proteolytic enzyme which breaks down proteins, to dissociate adherent cells from the vessel in which they are being cultured.

Growing RASMCs were harvested by trypsinization and collected into DMEM + 0.5% BSA + soybean trypsin inhibitor.

After two hours, the cells were harvested by trypsinization, and total RNA was extracted using TRIzol reagent (Gibco BRL, Gaithersburg, MD).

Trypsinization is often done to permit passage of the cells to a new container, observation for experimentation, or reduction of the degree of confluency in the flask by removal of a percentage of the cells.

After electroporation, cells were either plated directly on multiwell plates for harvest at short time periods (typically 4 days or less) or on tissue culture dishes for trypsinization and seeding onto multiwell plates one or two days before final harvest for longer time periods.

The finding of at least one closely apposed pair of cells expressing high levels of replicon proteins on day 9 post transfection, two days after trypsinization and replating (Figure 7, right panel), not only implies replicon replication, but also implies the expression of NS-1 protein as well.

The chain ganglia were incubated with 0.25% trypsin (Sigma) for 20 minutes at 37 C. Trypsinization was subsequently blocked by exposure to 100% heat-inactivated fetal bovine serum (Harlan Bioproducts for Science, Indianapolis, IN) for 5 minutes and washed 2 times with Ham's F12 medium.