thymocyte

The medulla is the location of the latter events in thymocyte development.

CD117 also identifies the earliest thymocyte progenitors in the thymus.

This protein plays a regulatory role in both positive and negative T cell selection during late thymocyte development.

A thymocyte's fate is determined during positive selection.

Acquired T cell deficiencies can also affect thymocyte development in the thymus.

The apoptosed thymocyte dies and is quickly recycled.

Taken together, our results further define the role TECs play at this complex stage of thymocyte development.

Discussion In this study, we have examined thymocyte proliferation in the fetal and adult wild type thymus.

In this study, we found stage-specific changes in thymocyte proliferation at the DP stage.

Thymocyte differentiation undergoes two main transitions.

Success in positive selection allows the thymocyte to undergo a number of maturational changes during the transition to a single positive T cell.

We also found distinct patterns of thymocyte proliferation in the Rag1 -/-and Hoxa3,Pax1 mutants.

A CD8/Lck transgene is able to drive thymocyte differentiation.

Therefore, blocks at different stages of thymocyte development result in differential effects on TEC differentiation and function.

Unfortunately, Hoxa3 +/- Pax1 -/-mice die at birth, preventing analysis of thymocyte proliferation in adult compound mutants.

For Southern blot analysis, thymocyte and brain DNA was isolated according to ref. 28.

In addition to proliferation, differentiation and T lineage commitment occurs within the DN thymocyte population.

The cortex is the location of the earliest events in thymocyte development, where T cell receptor gene rearrangement and positive selection takes place.

Because the expression of hCARΔcyt was detected early in thymocyte development (Fig.

The likelihood of this happening would obviously depend on the number of dendritic cells that are usually contacted by a thymocyte during thymic selection.

FIG. 2 Thymocyte numbers.

Our results showed clear stage-specific differences in wild type thymocyte proliferation depending on the developmental age of the microenvironment (i.e. fetal vs. adult).

The skewed pattern of thymocyte development toward CD4 +single positive in each of the lines was similar to that observed in DO11.10.

Once a mature thymic environment is formed, many, if not all stages of thymocyte maturation require interactions with thymic epithelial cells.

At the earliest TN1 stage, thymocyte proliferation levels in Rag1 -/-thymi were similar to fetal wild type cells (Fig.