thermal cycler

Instead he hung up the phone and turned back to the first thermal cycler tray.

With the first thermal cycler fully loaded, Sean turned it on.

Sean didn't know if he'd taken them from the thermal cycler after an in- sufficient number of cycles or if they were truly negative.

Place PCR tubes in the thermal cycler to begin cycling.

He loaded samples of the DNA into the remaining wells of the first thermal cycler.

The thermal cycler heats and cools the reaction tubes to achieve the temperatures required at each step of the reaction (see below).

The laboratory would add only the Taq polymerase enzyme and the specimen, and then place the tube into the thermal cycler.

"While I continue processing these thermal cycler samples, you look at the re- ferring histories on these thirty-three charts we copied.

Turning to the second thermal cycler, Sean added samples of Helen's tumor cell RNA to each well.

It combines the functions of a thermal cycler and a fluorimeter, enabling the process of real-time PCR.

Place PCR tube in thermal cycler for one cycle that includes annealing, extending and then inactivating reverse transcriptase.

The thermal cycler is also able to rapidly heat and chill samples, thereby taking advantage of the physicochemical properties of the nucleic acids and DNA polymerase.

This process amplifies genomic DNA samples using multiple primers and a temperature-mediated DNA polymerase in a thermal cycler.

Techne Ltd. was a company founded in Cambridge, UK by Norman de Bruyne in 1948 to manufacture gelation timers and thermal cycler equipment.

While the enzymes worked on the tumor sample, Sean be- gan loading some of the ninety-six wells of the first thermal cycler with aliquots of Helen's cerebrospinal fluid.

Later, the PCR process was adapted to the use of thermostable DNA polymerase from Thermus aquaticus, which greatly simplified the design of the thermal cycler.

In 1995, upon Andre Marion retirement, Mike Hunkapiller became President of PE Applied Biosystems Division which sold its 30,000th thermal cycler.

One long term goal of this project is to send a robotic thermal cycler with these primers to Mars in search of microbial life that is ancestrally related to life on Earth.

The advantages of HDA is that it provides a rapid method of nucleic acid amplification of a specific target at an isothermic temperature that does not require a thermal cycler.

Hence, fluorescence detected in the real-time PCR thermal cycler is directly proportional to the fluorophore released and the amount of DNA template present in the PCR.

Quantitative PCR is carried out in a thermal cycler with the capacity to illuminate each sample with a beam of light of a specified wavelength and detect the fluorescence emitted by the excited fluorochrome.

One early example is the polymerase chain reaction (PCR) which is able to amplify DNA strands using a thermal cycler to micromanage DNA synthesis by adjusting temperature using a pre-made computer program.

The thermal cycler (also known as a thermocycler, PCR machine or DNA amplifier) is a laboratory apparatus most commonly used to amplify segments of DNA via the polymerase chain reaction (PCR).

Despite the selling point that HDA negates the use of a thermal cycler and therefore allows research to be conducted in the field, much of the work required to detect potentially hazardous microorganisms is carried out in a research/hospital lab setting regardless.

To encode a PCR reaction using this method the protocol could describe the thermal cycling conditions and the make/model of the instrument, while the protocol application might give the sequences of the primers and the serial number of the thermal cycler.