sulfanilic acid

An accurate and simple method is proposed for the determination of sulfanilic acid in the presence of sulfonamides.

Sulfanilic acid can be produced by sulfonation of aniline:

Keywords: spectrophotometry, sulfanilic acid, sulfonamides, potassium 1,2-napthoquinone-4-sulphonate.

Sulfanilic acid. a) Fisher Scientific Cat.

Sulfonation of aniline produces p-aminobenzenesulfonic acid or sulfanilic acid which is a zwitterion with an unusual high melting point.

The compound reacted positively to ninhydrin, ammoniacal silver nitrate, bisdiazotized benzidine, and diazotized sulfanilic acid.

When proteins containing either tyrosine or histidine are reacted with diazotized sulfanilic acid under alkaline conditions, a red color is formed by a coupling reaction.

Alkaline coupling of anhydrotetracycline with diazotized sulfanilic acid gives an azo dye which is reduced by sodium dithionite to give 7-aminoanhydrotetracycline (9).

Sulfanilic acid (4-aminobenzenesulfonic acid ) is an off-white crystalline solid which finds application in quantitative analysis of nitrate and nitrite ions.

Azobilirubin is the red-violet compound formed by the condensation of diazotized sulfanilic acid with bilirubin in the van den Bergh reaction.

Sulfanilic acid Acetic acid (15% aqueous solution of glacial acetic acid) Complete Medium:

For example, reaction of aniline with sulfuric acid at 180 C produces sulfanilic acid, HNCHSOH.

Phloroglucinol, azure B, diazotized salts of p-nitroaniline and sulfanilic acid, and chlorine sulfite allowed visualization of phenolic localization but were not quantitative.

This method is based on measuring the intensity of the red colour that develops when sulfanilic acid is allowed to react with potassium 1,2-naphthoquinone-4-sulphonate (NS) in a chloroaceticchloroacetate buffer at pH 3,4.

Nitrite is detected and analyzed by the Griess Reaction, involving the formation of a deep red-colored azo dye upon treatment of a NO-containing sample with sulfanilic acid and naphthyl-1-amine in the presence of acid.

Nitrate is reduced to nitrite on a spongy cadmium column (the nitrite originally present is unaltered); sulfanilic acid is diazotised by the nitrite and coupled with N-(1-naphthyl)-ethylenediamine dihydrochloride to form a pink azo dye the absorbance of which is measured at 550 nm.