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Keep track of additions, because you will want to scale this up for a stock solution.
Other values may be used to determine a precise concentration of a stock solution.
Stock solutions for calibration are available through several commercial vendors.
Make up stock solutions and store in separate bottles with appropriate label.
Buffers were prepared as 2x or 5x stock solutions.
Stock solutions of test compounds were prepared in absolute alcohol and stored at -20 C until use.
This stock solution can be diluted 50:1 with water to make a 1X working solution.
Each aliquot was made up into a stock solution by dissolving in sterile water.
Both the stock solution and the complete Zenker fixative are stable for many years.
Stock solutions of inhibitors were made and serially diluted in the culture media.
Retinoic acid was freshly prepared as a 10mM stock solution in ethanol.
Both media have been modified as described in ref. 54 and are made up from stock solutions given in Table 5.
The stock solution is then diluted with a 9:1 ratio of absolute ethyl alcohol.
A stock solution of the correct concentrations of contaminant must be previously prepared.
The stock solution for Karnovsky fixative is as follows:
"Are those stock solutions you're mixing?"
TAE buffer is commonly prepared as a 50X stock solution for laboratory use.
Stock solutions do not necessarily come in concentrations of simple numbers; for example a solution could be 0.10082 M HCl.
The methanolic stock solution is stable and also serves the purpose of directly fixing the smear eliminating a prefixing step.
Stock solution may be made up in tap water though 'purified water' is preferred as helps with the short stability of the working solution.
However, concentrated stock solutions may precipitate when cooled and should be kept at room temperature until precipitate has completely dissolved before use.
Stock solutions were prepared by dissolving the inhibitors in DMSO.
Additional specimens were taken from the stock solution, and diluted and plated to confirm the counts estimated by spectrophotometry.
A Stock Solution is a concentrated solution that will be diluted to some lower concentrated for actual use.
Phosphate buffered saline was freshly prepared before staining from frozen 10x stock solution, and pH adjusted to 7.22.