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The specific radioactivity of each fatty acid in each fraction could then be calculated.
Specific radioactivity S is defined as radioactivity per unit mass of the radionuclide:
For direct comparison, each chemokine was added at the same specific radioactivity and concentration, and autoradiography was performed for an identical exposure time.
Results were expressed as nmol substrate metabolised per hour, after correcting for specific radioactivity of the substrate and number of 1 4 C atoms per molecule.