site-directed mutagenesis

Site-directed mutagenesis is a technique that has been around since the 1970s.

Specific changes are then made by site-directed mutagenesis in an attempt to change the function of the protein.

I use both patch-clamping and site-directed mutagenesis to achieve this.

Site-directed mutagenesis can be useful for many different reasons.

Westheimer et al.'s proposal was further supported by site-directed mutagenesis studies.

Mutations can be introduced into any region of interest in the channel protein using site-directed mutagenesis.

It was found to be a non-coding transcript through site-directed mutagenesis experimentation.

This online tool is being developed in such a way that it can be applied to any system for simulated site-directed mutagenesis.

Our aim is to determine the sites essential for ion transfer using site-directed mutagenesis.

Myc point mutants were generated by site-directed mutagenesis using standard methods.

Site-directed mutagenesis selectively introduces mutations that change the structure of a protein.

The structure/function relationships of a number of the proteins are being investigated by site-directed mutagenesis.

In general, this has the advantage of being inexpensive and technically easy, since site-directed mutagenesis techniques are well-developed.

This is often investigated through site-directed mutagenesis.

Is has also enabled us to use site-directed mutagenesis to analyse structure function relationships in cytochrome P450scc.

His cloning vectors were also used to develop the method for oligonucleotide site-directed mutagenesis.

Site-directed mutagenesis was done using the phosphothioate method (Amersham).

The contribution of this lid to the activity of Hsp90 has been probed with site-directed mutagenesis.

This technique, called "oligonucleotide-directed site-directed mutagenesis", showed how to find the effect of a single mutant gene.

Today phagemids are still useful for generating templates for site-directed mutagenesis.

Data from over 50 experiments are presented including gene inactivations, site-directed mutagenesis, and promoter exchanges.

The involvement of one of these two regions, RelA-2, is consistent with a previous site-directed mutagenesis study.

In addition, site-directed mutagenesis (molecular biology) will be used to determine which residues/domains are important for these interactions.

We provide both site-directed mutagenesis (Fig.

We can use site-directed mutagenesis to correct clones that carry single nucleotide changes or other small, localized defects.