In J99 and 87A300, orf7 contains an insertion in the coding sequence.
They should be 21mers, be at least 25bp from start of the coding sequence and no closer than 150bp from its end.
The positions of exons within the coding sequence can be used to infer common ancestry.
With these settings, 5.7% of all non coding sequences are masked.
Hgpr84 locates to chromosome 12q13.13, and its coding sequence is not interrupted by introns.
The coding sequence is from base pairs 151 to 624.
The mutation is a transition from a cytosine to a thymidine at base position 1429 in the coding sequence.
"I have the coding sequence in my inside coat pocket."
Further analysis of this limited number of unique coding sequences will be critical in developing specific diagnostic reagents.
Exon 7 is the largest and contains most of the coding sequence.