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However, sedimentation analysis indicates that the insertion mutants form unstable dimer.
Preliminary sedimentation analysis yielded a sedimentation coefficient of 9.5 for this enzyme.
Sedimentation analysis showed that loss of catalytic activity in these denaturing media is accompanied by dissociation into slower sedimenting subunits.
Analytical ultracentrifuges are designed to perform sedimentation analysis of macromolecules using the principles devised by Theodor Svedberg.
By sedimentation analysis of their alkaline lysates in alkaline sucrose gradients, a plasmid was found in four strains.
Brezina, Jiri, 1989, Sand sedimentation analysis and separation - 25 years of research and development (in German); Deutsche Geologische Gesellschaft Nachrichten, vol.
Our glycerol gradient sedimentation analysis had revealed that the p66 WT/p51 INSmutant does not form stable dimers, rather the p66 WTsubunit tends to self dimerize.
Based on our observations from the sedimentation analysis, we propose that the loss of DNA binding function in case of the HIV-1RT insertion mutants results from a failure to form stable dimers.
Ribosomes were first visualized using electron microscopy, and their ribonucleoprotein components were identified by biophysical methods, chiefly sedimentation analysis within ultracentrifuges capable of generating very high accelerations (equivalent to hundreds of thousands times gravity).
Brezina, Jiri, 1971b, Investigations on an instrument for sedimentation analysis in the particle range above 60 um: Report about research studies of the author at the Institute for Mechanical Engineering, University of Karlsruhe (1968-1971).
Results Glycerol gradient ultra-centrifugation analysis The ability of the HIV-1 RT mutants, carrying insertion of six residues in the β7-β8 loop in either one or both the subunits was analyzed by glycerol gradient sedimentation analysis.