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Ribosomes are most often identified by their sedimentation coefficient.
By convention, all sedimentation coefficients are expressed in the Svedberg units.
Values of sedimentation coefficient (S) can be calculated.
The K-factor is related to the sedimentation coefficient by the formula:
The sedimentation coefficient s also equals , where is the buoyant mass.
The sedimentation coefficient has the dimensions of a unit of time and is expressed in svedbergs.
Bigger particles sediment faster and have higher sedimentation coefficients (svedberg, or S values).
Sedimentation coefficients are, however, not additive.
Ribosomal particles are denoted according to their sedimentation coefficients in Svedberg units.
Hence, it is generally possible to define a sedimentation coefficient that depends only on the properties of the particle and the surrounding fluid.
For instance, the 70 S ribosome that comes from bacteria actually has a sedimentation coefficient of 70 svedberg.
Errors in molecular mass were propagated from standard deviations for Stokes radius and sedimentation coefficient measurements.
Sedimentation coefficients quoted in literature usually pertain to sedimentation in water at 20 C.
The sedimentation coefficient s of a particle is used to characterize its behaviour in sedimentation processes, notably centrifugation.
We define the Mason-Weaver sedimentation coefficient .
We carried out velocity sedimentation of Myo1c-calmodulin complexes on 5-20% sucrose gradients to determine sedimentation coefficients.
The sedimentation coefficient is the ratio of the speed of a substance in a centrifuge to its acceleration in comparable units.
The proteasome subcomponents are often referred to by their Svedberg sedimentation coefficient (denoted S).
It can be used to estimate the time (in hours) required for sedimentation of a fraction with a known sedimentation coefficient (in svedbergs):
When the sedimentation coefficient, s, of circular DNA is ascertained over a large range of pH, the following curves are seen.
Eukaryotic ribosomes have two unequal subunits, designated small subunit (40S) and large subunit (60S) according to their sedimentation coefficients.
One svedberg is defined as exactly 10 s. Essentially the sedimentation coefficient serves to normalize the sedimentation rate of a particle by the acceleration applied to it.
These Eukaryotic ribosomes are also known as 80S ribosomes, referring to their sedimentation coefficients in Svedberg units, because they sediment faster than the prokaryotic (70S) ribosomes.
Although most experiments used 400 mM KCl (which prevented adsorption to the gel-filtration matrix), we obtained identical sedimentation coefficients in the presence of 150 or 250 mM KCl (not shown).
To calculate the molecular mass of Myo1c-calmodulin complexes, we applied the modified Svedberg equation, which relates mass to the diffusion constant (calculated here from Stokes radius) and the sedimentation coefficient [ 29 ] .