real-time polymerase chain reaction

There are numerous applications for real-time polymerase chain reaction in the laboratory.

It is based on the biological enrichment of a sample followed by a real-time polymerase chain reaction assay.

Internal control of techniques used in molecular biology, such as western blot and real-time polymerase chain reaction.

The real-time polymerase chain reaction.

Real-time polymerase chain reaction quantification of canine DNA.

Rapid and specific diagnosis of the virus can be accomplished using nested or real-time polymerase chain reaction (PCR).

The model was validated by real-time polymerase chain reaction (RT-PCR) and indicates good concordance between the two experimental techniques.

Quantitative real-time polymerase chain reaction technology (qRT-PCR) confirmed 68 of the candidate genes from which the 20-gene gene expression panel was selected.

RT-PCR is often confused with real-time polymerase chain reaction (qPCR) by students and scientists alike.

PrimerDesign is a UK based biotechnology company that designs and sells products for quantitative real-time polymerase chain reaction (qPCR).

Real Time PCR analysis The amount of adenoviral genomes per cell was determined by a multiplex real-time polymerase chain reaction [ 22 ] .

The RFU measurements are used, for DNA profiling, in a real-time polymerase chain reaction (PCR).

The test is based on standard quantitative real-time polymerase chain reaction technology (qRT-PCR) using RNA isolated from peripheral blood mononuclear cells (PBMC).

Fluorescence-based thermal shift assay can be performed on instruments that combine sample temperature control and dye fluorescence detection, such as readily available real-time polymerase chain reaction (RT-PCR) instrumentation.

Over the past decade, real-time polymerase chain reaction (PCR) methods have been used to detect the presence of various microbial pathogens through the amplification of specific DNA sequences without culturing bacteria.

The "gold standard" for determining toxin type is a mouse bioassay, but the genes for types A, B, E, and F can now be readily differentiated using Real-time polymerase chain reaction (PCR).

Real-time polymerase chain reaction (RT-PCR) The results obtained from a microarray experiment are influenced by each step in the experimental procedure, from array manufacturing to sample preparation and application to image analysis [ 25 ] .

According to the Baby Gender Mentor web site, the test involves using real-time polymerase chain reaction and a proprietary technology to detect markers on the Y-chromosome, which can be isolated from the pregnant woman's blood if the fetus is male.

As the global reference laboratory for measles, provides resources for national reference laboratories and provides technical assistance to other global public health laboratories for the collection and shipment of clinical samples for measles RT-PCR (real-time polymerase chain reaction).

Therefore, it is common practice in protein quantification studies (western blot) and transcription studies (Real-time polymerase chain reaction) to carry out the quantification of the gene of interest and also the quantification of a reference gene such as the one that codes for actin.

Laboratory diagnosis of TOSV infection can be performed through the use of ELISA, immunofluorescence and/or neutralization tests, but reverse transcription, real-time polymerase chain reaction assays are preferred because they are less time-consuming and reduce the risk of contamination.

In molecular biology, real-time polymerase chain reaction, also called quantitative real time polymerase chain reaction (qPCR) or kinetic polymerase chain reaction is a laboratory technique based on the polymerase chain reaction, which is used to amplify and simultaneously quantify a targeted DNA molecule.