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They also does not allow ratiometric measurements.
However, we have used dextran coupled indicator dyes and a ratiometric method.
Ratiometric micro-ohmmeter for measuring resistance using the four-point method.
Measurement Method: Full ratiometric with automatic compensation for excitation offsets.
Fluorescence photometry equipment for high-speed measurement of ratiometric dyes.
Fura-2, an aminopolycarboxylic acid, is a ratiometric fluorescent dye which binds to free intracellular calcium.
It offered Filtered Ratiometric Expansion, which automatically adjusted images to full-screen size.
The ratiometric data were converted to pH using a calibration curve from the lysosomes of ionophore-clamped cells.
Its output is a fully push-pull 12-bit ratiometric analog output with less than 25 mV of noise.
Images are analyzed by measuring fluorescence intensity changes for a single wavelength or two wavelengths expressed as a ratio (ratiometric indicators).
Most interestingly, a heterogeneous distribution of lysosomes with elevated pH was observed by ratiometric images as shown in Figure 4.
The fuel level output for these senders can be ratiometric voltage or preferable CAN bus digital.
Ratiometric measurements (light in versus light out) from 400 to 1000 nm are useful for measuring chromophores and light scattering.
We have investigated the responsiveness of adult guinea pig Schwann cells to a range of neuroligands, using ratiometric calcium imaging.
If you need a fast fluorescence system, for example for ratiometric ion measurement, then photometry may be an economical alternative to using a fast camera.
The basic geometry of ratiometric capacitative transducers consists of fixed cylindrical electrodes with a co-axial moving screen.
Piezoresistive transducers configured as Wheatstone bridges often exhibit ratiometric behavior with respect not only to the measured pressure, but also the transducer supply voltage.
FLIM imaging is particularly useful in neurons, where light scattering by brain tissue is problematic for ratiometric imaging.
To circumvent the potential problem of fluorescence artifact, we utilized confocal laser scanning microscopy to image intracellular Ca2+ movements with the ratiometric fluorophore Indo-1.
All transitions are glitchless, and the parts are extremely stable with a nominal ratiometric resistor temperature coefficient of 5ppm/° C. Power consumption is just 100nA.
MetaFluor supports FURA-2, BCECF, INDO-1 and other common ratiometric indicators.
Clomeleon and Cl Sensor are FRET-based Cl indicators that allow ratiometric non-invasive monitoring of chloride activity in living cells.
CaMPARI (calcium-modulated photoactivatable ratiometric integrator) is a fluorescent protein whose fluorescence permanently changes from green to red upon UV irradiation in presence of calcium.
Averaged lysosomal pH was measured in control cells or cells treated with PMA, using fluorescein-conjugated dextran in dual-excitation ratiometric mode [ 8 ] .
This allows ratiometric Cl measurements based on the Cl sensitivity of YFP and Cl insensivity of CFP.