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After gentle pipetting, cells floating in the medium were collected.
Sean turned back to his lab bench and his pipetting.
Most pipetting can be performed in the Eppendorf tube protector.
The viral pellet was resuspended in fresh medium by gentle pipetting.
Gentle pipetting then released these cells leaving the remainder of the rudiment largely intact.
This process can be hastened by gentle pipetting of the egg masses through a wide bore glass mouth pipette.
Mouth pipetting is now considered unsafe due to the possibility of accidentally ingesting or inhaling toxic chemicals or pathogens.
The gel stays for the whole procedure in this reaction chamber, only the different buffers are added by manual pipetting and centrifugation and removed by centrifugation.
(iv) Remove these outer cells by pipetting the embryos through a flame-polished micropipette with an internal diameter slightly larger than the clump of inside cells or ICM.
Dispensing fluids by manual or robotic pipetting can be replaced with micropumps and microvalves, where fluid metering is straightforward to determine as opposed to continuous flow systems by micromixers.
The word "pipetting" is referring to the laboratory manual tool called pipette, which is the commonly used in molecular biology, analytical chemistry, and medical tests, to transport a measured volume of liquid.
After final removal of supernatant, the pellet was air dried for 1-2 minutes and dissolved in nanopure H 2 O by gentle pipetting and, where necessary, by incubation at 37 C for 10 minutes.