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In these instances, the region of photobleaching is within the nucleus.
Measured photobleaching was well described by a model based on quantum yields.
Another way to minimize error is to keep photobleaching contained to a single region or area.
Photobleaching is the name for the destruction of fluorophores by high intensity light.
A number of initial scans need to be made to determine fluorescence before photobleaching.
Unfortunately, this high intensity laser can lead to the issue of photobleaching the fluorophore.
The implications of this model for photobleaching experiments in liquids are briefly discussed.
Fluorophores lose their ability to fluoresce as they are illuminated in a process called photobleaching.
Remember, you cannot simply correct for photobleaching mathematically.
Photobleaching can be used to determine cone arrangement.
As with most fluorescence techniques, a significant problem with immunofluorescence is photobleaching.
Attenuation of photobleaching in the water column was similar to that of blue irradiance.
How much of the QR is present determines the intensity of the photobleaching.
The disadvantage of the fluorospheres is photobleaching.
The single arrow shows a single molecule which undergoes a single photobleaching step.
Photobleaching can severely limit the time over which a sample can be observed by fluorescent microscopy.
A common problem in fluorescence microscopy is photobleaching, where the fluorophore loses its activity as a result of exposure to light.
Photobleaching can then occur.
These characteristics drive other properties, including the photobleaching or photoresistance (loss of fluorescence upon continuous light excitation).
Also, the greater the amount of time QR is exposed to intensive rays, the more the substance will undergo photobleaching.
FISH reagents fade overtime due to photobleaching so a sample can only be examined once.
LEDs can be adjusted and controlled, and the chance of photobleaching or phototoxicity is reduced.
Furthermore, for samples that are sensitive to photobleaching, lower excitation intensity can be used hence allowing for longer imaging times.
Too high and the cell will suffer photobleaching and possible death, however, the carbohydrate produced during photosynthesis increases the cell's density, causing it to sink.
However, photobleaching may also be used prior to applying the (primarily antibody-linked) fluorescent molecules, in an attempt to quench autofluorescence.