phosphate buffer

Adding phosphate buffer to mastoparan resulted in no effect.

Finally, preparations were extensively dialyzed against phosphate buffer to remove free glucose.

Washington, PA) in 0.1 M phosphate buffer for approximately twenty minutes.

Tongues were then put into a 20% sucrose solution in 0.1 M phosphate buffer overnight for cryoprotection.

Twenty-four hours prior to sectioning, the tissue was exposed to a solution of phosphate buffer containing 20% sucrose.

Cryostat sections (20 microns) from spinal cord and brain were cut and stored in phosphate buffer.

PLE hydrolyses are typically carried out with a phosphate buffer to maintain the pH between 7 and 8.

The resin is then washed with phosphate buffer to remove proteins that do not specifically interact with the cobalt or nickel ion.

The remaining pellet was resuspended in 3 ml of 50 mM phosphate buffer (pH 7.0) and sonicated to yield the cytoplasmic fraction.

After washing in phosphate buffer saline (PBS), aliquots of 1x10 4 cells were added to a six well tissue culture plate (Gibco).

This compound can be hydrolyzed under basic conditions in a phosphate buffer to nitroxyl HNO, acetic acid and cyclohexanone.

The aaDNA is then purified with a column (using solution containing phosphate buffer as Tris contains amine groups).

The animals were deeply anesthetized with pentobarbital and transcardially perfused with 4% paraformaldehyde in 0.1 M phosphate buffer (pH 7.4).

In addition, apparent intracellular drug concentrations were estimated after each of the washes by ultrasonic disruption of the pellet in 0.05 M phosphate buffer, as above.

Incubation time is important since the different NONOates have different half-lives (t) in phosphate buffer at pH 7.4.

Immuno-electron microscopy Mice were perfused under anesthesia with fixative (4% paraformaldehyde, 0.1% glutaraldehyde in 0.1 M phosphate buffer, pH 7.5).

The concentrated preparation was dialyzed overnight against 50 mM phosphate buffer (pH 7.0) containing 1 mM DTT.

The eluate is collected into a neutral tris or phosphate buffer, to neutralize the low pH elution buffer and halt any degradation of the antibody's activity.

Field's stain uses methylene blue and Azure 1 dissolved in phosphate buffer solution, and Eosin Y in buffer solution.

The samples were then placed into microcentrifuge tubes containing 1 mL of sodium phosphate buffer (10 mmol/L, pH 7.4) and finely minced with scissors for 15 seconds.

After washing in phosphate buffer, tissues were osmicated and dehydrated in acidified DMP before routine embedding in Taab resin.

When organic soil was used, citrate buffer in the extraction mixture gave higher amounts of lipid phosphate than acetate buffer,Tris,H2O or phosphate buffer.

Instead of collecting onto a greased substrate or agar plate, impingers have been developed to impact bioaerosols into a liquid, such as deionized water or phosphate buffer solution (PBS).

Fractions showing high chorismate mutase activity were pooled and dialyzed overnight against the above phosphate buffer containing 1 mM DTT and 150 mM NaCl.

Cells were harvested in the late-exponential phase of growth by centrifugation and resuspended in 50 mM potassium phosphate buffer (pH 7.0), containing 1 mM dithiothreitol (DTT).