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Frozen and paraffin embedded archival tissue may also be used.
Paraffin embedded and frozen sections were prepared as needed.
It uses paraffin embedded or fresh tissue for the microarray analysis.
This antibody can be used on paraffin embedded tissue.
One to six paraffin embedded blocks were measured for each patient (mean 2.5 blocks).
However, paraffin embedded tissues have limitations.
All human tissues were formalin fixed, paraffin embedded and were previously used for diagnostic purposes.
Array-based karyotyping performs well on paraffin embedded tumors and is amenable to routine clinical use.
All slices involved with tumor were paraffin embedded, sliced again into 4 μm slides, and stained with hematoxylin-eosin.
HER2 amplification can be detected by virtual karyotyping of formalin-fixed paraffin embedded tumor.
The quality of the slides produced by frozen section is of lower quality than formalin fixed paraffin embedded tissue processing.
All axillary lymph nodes were paraffin embedded, sliced into 4 μm slides and assessed for the presence of micrometastases.
DNA ploidy was analysed by flow cytometry on nuclei isolated from paraffin embedded tumour tissue.
For stages E11.5 through P6, whole heads were fixed in Bouin's solution, paraffin embedded and sectioned at 5 μm thickness.
Formalin fixed, paraffin embedded tumour tissue was prepared and stained for flow cytometric analysis according to the slightly modified procedure described by Hedley et al.
Furthermore, because of the higher coefficient of variation paraffin embedded material cell cycle analysis is not as reliable as in fresh tissue of solid tumours.
paraffin embedded tissue preparations
A recent study has demonstrated the success of MIP for copy number variation and genotyping studies in formalin-fixed paraffin embedded samples.
The study, however, successfully applied MIP to obtain high quality copy number and genotyping data from formalin-fixed paraffin embedded samples.
Monosomy 3, along with other chromosomal gains, losses, amplifications, and LOH, can be detected in fresh or paraffin embedded samples by virtual karyotyping.
Toxoplasma gondii infection of the central nervous system: use of the peroxidase-antiperoxidase method to demonstrate toxoplasma in formalin fixed, paraffin embedded tissue sections.
The best diagnostic method currently available is Polymerase chain reaction (PCR), which can be performed in fresh sample or paraffin embedded formalin fixed tissue samples.
Paraffin embedded sections were stained for conventional study with haematoxylin-eosin-safran (HES) and periodic acid Schiff (PAS).
Use of alcian blue has historically been a popular staining method in histology especially for light microscopy in paraffin embedded sections and in semithin resin sections.
Genome-wide LOH status of fresh or paraffin embedded tissue samples can be assessed by virtual karyotyping using SNP arrays.