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His further research centered around canine nutrition, fluoride and its use in water treatment, and parabiosis.
"Heterochronic parabiosis: historical perspective and methodological considerations for studies of aging and longevity."
He is remembered for his research involving limb regeneration and parabiosis of the Mexican axolotl (Ambystoma mexicanum).
Surgery to separate conjoined twins has allowed surgeons to be able to study the mechanisms of embryogenesis as well as the physiological consequences of parabiosis.
GDF11 gene expression and protein abundance decreases with age, and it shows differential abundance between young and old mice in parabiosis procedures, causing youthful regeneration of cardiomyocytes, a reduction in the Brain natriuretic peptide (BNP) and in the Atrial natriuretic peptide (ANP).